| 00:00:00 | Welcome |
| 00:00:09 | Welcome |
| 00:02:02 | Microcalorimetry of nanoparticles |
| 00:02:18 | Outline |
| 00:03:31 | Nanotechnology and nanoparticles |
| 00:04:22 | Nanotechnology and nanoparticles |
| 00:04:54 | Nanotechnology and nanoparticles |
| 00:06:43 | Nanoparticles in biomedical research |
| 00:08:13 | Overview of nanoparticles and applications in life sciences/biomedicine |
| 00:08:48 | Design of nanoparticles for drug delivery |
| 00:10:09 | Analytical methods to characterize nanoparticles |
| 00:11:12 | Why microcalorimetry for characterization of nanoparticles and biomolecules? |
| 00:13:51 | Microcalorimetry systems from Malvern Instruments |
| 00:14:23 | What is Isothermal Titration Calorimetry (ITC)? |
| 00:15:35 | Performing an ITC assay |
| 00:17:27 | Basics of ITC experiment |
| 00:20:15 | The energetics |
| 00:21:41 | ITC characterizes a broad range of interactions |
| 00:22:02 | Isothermal titration calorimetry and nanoparticles |
| 00:23:13 | How ITC is used in nanoparticle characterization |
| 00:24:06 | Isothermal Titration Calorimetry Studies on the Binding of Amino Acids to GoldNanoparticles |
| 00:25:14 | Binding of aspartic acid to gold nanoparticles |
| 00:27:38 | Binding of lysine to gold nanoparticles |
| 00:29:01 | Conclusions |
| 00:29:37 | Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles |
| 00:30:42 | ITC of HSA and nanoparticles |
| 00:32:02 | Conclusions |
| 00:32:42 | Trapping RNase A on MCM41 pores: effects on structure stability, product inhibition and overall enzymatic activity |
| 00:34:07 | ITC of absorption of RNase on MCM41 |
| 00:34:57 | Enzyme catalysis by ITC – RNase in absence and presence of MCM41 |
| 00:37:24 | Conclusions |
| 00:37:59 | Elucidating the molecular mechanism of PAMAM–siRNA dendriplex self-assembly: Effect of dendrimer charge density |
| 00:38:46 | ITC of siRNA with dendrimers |
| 00:40:15 | Conclusions |
| 00:41:03 | Enhanced Antitumor Activity of the Photosensitizer meso-Tetra(N‑methyl-4-pyridyl) Porphine Tetra Tosylate through Encapsulation in Antibody Targeted Chitosan/Alginate Nanoparticles |
| 00:42:09 | ITC of TMP and alginate |
| 00:43:00 | Conclusions |
| 00:43:14 | Formation of nanoparticles by cooperative inclusion between (S)-camptothecin-modified dextrans and β-cyclodextrin polymers |
| 00:44:44 | Formation of polymer-β-CD complex by ITC |
| 00:45:27 | Conclusions |
| 00:46:09 | Peptide-Mediated Constructs of Quantum Dot Nanocomposites for Enzymatic Control of Nonradiative Energy Transfer |
| 00:47:16 | ITC of polyelectrolyte peptides |
| 00:47:54 | ITC data of peptide pairs |
| 00:48:39 | Conclusions |
| 00:48:58 | Isothermal titration calorimetry and nanoparticles |
| 00:49:49 | MicroCal VP-ITC system from Malvern Instruments |
| 00:50:32 | MicroCal iTC200 system from Malvern Instruments |
| 00:51:13 | Thank you!! |
| 00:57:00 | The energetics |
| 00:59:42 | Contact Information |
| 01:01:17 | Contact Information |
| 01:01:26 | More information |
In this webinar, find out how Isothermal Titration Calorimetry (ITC) can be used to characterize nanoparticles. Isothermal titration calorimetry (ITC) is used to characterize the thermodynamics of nanoparticle assembly, as well as nanoparticle interactions with proteins, membranes and other molecules. ITC is label free and can be used with nanoparticles in suspension.