Use of isothermal titration calorimetry | Malvern Panalytical

日期记录: April 13 2016

Duration: 22 minutes 02 seconds

The search for novel peptide substrates for proline-specific peptidases has been hampered by a lack of suitable assays. Existing technologies are often too expensive, labor-intensive or require derivatization and separation of the reaction products. The purpose of this study was to validate the use of isothermal titration calorimetry (ITC) to study enzyme kinetics, in comparison to more conventional methods (e.g. MALDI-TOF mass spectrometry, quantitation of released C-terminal Phe using RP-HPLC). The study led to the discovery of a novel substrate for prolyl carboxypeptidase, an enzyme involved in body weight regulation.

Table of contents

1. Use of isothermal titration calorimetry to investigate and identify novel peptide substrates for prolyl carboxypeptidase: a technology comparison

01:23

2. Use of isothermal titration calorimetry to investigate and identify novel peptide substrates for propyl carboxypeptidase.

00:17

3. Introduction

00:20

4. Introduction

00:13

5. Introduction

00:04

6. Introduction

00:10

7. Introduction

00:12

8. Introduction

00:18

9. Introduction

00:11

10. Aims

00:06

11. Aims

00:03

12. Aims

00:05

13. Aims

00:04

14. Aims

00:16

15. Aims

00:23

16. Aims

00:12

17. Isothermal Titration Calorimetry (ITC)

00:21

18. Isothermal Titration Calorimetry (ITC)

00:07

19. Isothermal Titration Calorimetry (ITC)

00:09

20. Isothermal Titration Calorimetry (ITC)

00:08

21. Isothermal Titration Calorimetry (ITC)

00:29

22. Isothermal Titration Calorimetry (ITC)

00:06

23. Isothermal Titration Calorimetry (ITC)

00:04

24. Isothermal Titration Calorimetry (ITC)

00:03

25. Isothermal Titration Calorimetry (ITC)

00:09

26. Isothermal Titration Calorimetry (ITC)

00:22

27. Isothermal Titration Calorimetry (ITC)

00:05

28. Isothermal Titration Calorimetry (ITC)

00:03

29. Isothermal Titration Calorimetry (ITC)

00:03

30. Isothermal Titration Calorimetry (ITC)

00:06

31. Isothermal Titration Calorimetry (ITC)

00:04

32. Isothermal Titration Calorimetry (ITC)

00:06

33. Isothermal Titration Calorimetry (ITC)

00:08

34. Isothermal Titration Calorimetry (ITC)

00:15

35. Isothermal Titration Calorimetry (ITC)

00:26

36. Isothermal Titration Calorimetry (ITC)

00:07

37. Isothermal Titration Calorimetry (ITC)

00:13

38. Isothermal Titration Calorimetry (ITC)

00:04

39. Isothermal Titration Calorimetry (ITC)

00:05

40. Isothermal Titration Calorimetry (ITC)

00:15

41. 1. Determine peptide and enzyme concentrations

00:17

42. 1. Determine peptide and enzyme concentrations

00:06

43. 1. Determine peptide and enzyme concentrations

00:19

44. 1. Determine peptide and enzyme concentrations

00:08

45. 1. Determine peptide and enzyme concentrations

00:06

46. 1. Determine peptide and enzyme concentrations

00:07

47. 1. Determine peptide and enzyme concentrations

00:16

48. 1. Determine peptide and enzyme concentrations

00:04

49. 1. Determine peptide and enzyme concentrations

00:03

50. 1. Determine peptide and enzyme concentrations

00:05

51. 1. Determine peptide and enzyme concentrations

00:05

52. 1. Determine peptide and enzyme concentrations

00:07

53. 1. Determine peptide and enzyme concentrations

00:04

54. 1. Determine peptide and enzyme concentrations

00:14

55. 1. Determine peptide and enzyme concentrations

00:11

56. 2. Determine total molar enthalpy

00:16

57. 2. Determine total molar enthalpy

00:10

58. 2. Determine total molar enthalpy

00:09

59. 2. Determine total molar enthalpy

00:05

60. 2. Determine total molar enthalpy

00:23

61. 2. Determine total molar enthalpy

00:06

62. 2. Determine total molar enthalpy

00:04

63. 2. Determine total molar enthalpy

00:11

64. 2. Determine total molar enthalpy

00:08

65. 2. Determine total molar enthalpy

00:06

66. 2. Determine total molar enthalpy

00:24

67. 3. Perform ITC experiment

00:10

68. 3. Perform ITC experiment

00:05

69. 3. Perform ITC experiment

00:08

70. 3. Perform ITC experiment

00:19

71. Results

00:04

72. 1. Hydrolysis of angiotensin III by rPRCP

00:07

73. 1. Hydrolysis of angiotensin III by rPRCP

00:19

74. 1. Hydrolysis of angiotensin III by rPRCP

00:10

75. 1. Hydrolysis of angiotensin III by rPRCP

00:11

76. 1. Hydrolysis of angiotensin III by rPRCP

00:14

77. 2. Hydrolysis of (pyr)-apelin-13 by rPRCP

00:07

78. 2. Hydrolysis of (pyr)-apelin-13 by rPRCP

00:07

79. 2. Hydrolysis of (pyr)-apelin-13 by rPRCP

00:07

80. 2. Hydrolysis of (pyr)-apelin-13 by rPRCP

00:11

81. 2. Hydrolysis of (pyr)-apelin-13 by rPRCP

00:09

82. 2. Hydrolysis of (pyr)-apelin-13 by rPRCP

00:12

83. 3. Method comparison

00:53

84. 3. Method comparison

00:28

85. 3. Method comparison

00:39

86. Conclusion

00:08

87. Conclusion

00:06

88. Conclusion

00:07

89. Conclusion

00:19

90. Acknowledgements

00:20

91. Acknowledgements

00:08

92. Thank you for your attentionAny questions?

01:54

93. Untitled

01:10

94. Thank you for your attentionAny questions?

00:25

95. Contact Information

00:16

联系销售部门现在注册