| 00:00:00 | Welcome |
| 00:01:03 | “The Sweet Smell of Success” – Understanding The Effect of Polysaccharide Molecular Weight and Structure on Final Product Performance |
| 00:01:43 | Outline |
| 00:02:41 | Natural polymers, The Applications |
| 00:03:52 | The drivers for better characterization |
| 00:05:39 | What are polysaccharide characterization challenges? |
| 00:05:46 | Measurement challenges |
| 00:07:31 | Sample challenges |
| 00:09:25 | How Can we address them? |
| 00:09:35 | OMNISEC |
| 00:10:03 | OMNISEC |
| 00:10:27 | OMNISEC REVEAL technology |
| 00:11:29 | Refractive index |
| 00:13:07 | UV/Vis PDA |
| 00:13:52 | Light scattering |
| 00:15:31 | PEO 196 Da by Triple Detection ! |
| 00:15:49 | Molecular weight vs. Molecular size |
| 00:17:36 | The importance of size in solution |
| 00:18:49 | Viscometer |
| 00:19:36 | OMNISEC Viscometer benefits |
| 00:20:56 | Viscometer technology |
| 00:21:23 | What can Advanced SEC provide scientists? |
| 00:22:34 | The multi-detection pyramid |
| 00:23:47 | OMNISEC RESOLVE design |
| 00:24:46 | Separation module – OMINSEC RESOLVE |
| 00:25:13 | Separation module – OMINSEC RESOLVE |
| 00:26:05 | Separation module – OMINSEC RESOLVE |
| 00:26:37 | Why is OMNISEC better? |
| 00:27:14 | OMNISEC software v10.0 |
| 00:27:54 | What are the results? |
| 00:28:00 | Cellulose derivatives |
| 00:28:39 | Modified celluloses - HEC |
| 00:29:19 | Molecular weight series of HECs |
| 00:29:48 | Cellulose derivatives |
| 00:31:18 | Native Cellulose |
| 00:32:14 | Triple Detection Overlay of Two Cellulose Samples |
| 00:33:10 | Structural Comparison of Two cellulose samples from different sources |
| 00:34:11 | Dextran |
| 00:34:41 | Dextran Series |
| 00:35:24 | Dextran Series – Mark-Houwink Plots |
| 00:36:49 | Pectin |
| 00:37:06 | Pectins (citrus, apple) |
| 00:38:13 | Hyaluronic Acid |
| 00:39:34 | Hyaluronic Acid – Linear and Crosslinked |
| 00:41:01 | Polysaccharide Mark-Houwink plots |
| 00:41:38 | Summary |
| 00:41:42 | Measurement challenges |
| 00:43:34 | Sample challenges |
| 00:44:19 | What does advanced SEC offer? |
| 00:45:02 | Malvern SEC range |
| 00:45:55 | Thanks for listening |
| 00:54:43 | Contact Information |
From simple food additives to complex material used in high-tech eye surgery and targeted drug delivery systems, polysaccharides have earned unparalleled attention when it comes to macromolecular characterization. With more emphasis on product performance, versatility and stability; detailed characterization is the key to understanding important parameters that affect the quality of these products. Simple techniques stand unable to provide the answers needed.
Gel Permeation Chromatography (GPC), or Size Exclusion Chromatography (SEC), is the technique of choice for rapid and reliable determination of molecular weight and molecular structure for all types of macromolecules. Furthermore, in the last 20 years there has been a significant change to the way GPC/SEC has been used in polymer and biologics analysis.
Measurement of polysaccharide molecular weight, size, branching and intrinsic viscosity can highlight the differences in product performance due to a change in polymer structure and those differences that are caused by a change in molecular weight.
This webinar will focus on the advancements in light scattering and on-line viscometry techniques and the benefits they provide to the technique of polysaccharide characterization by GPC/SEC. The fundamentals of these measurements will also be discussed and illustrated with application examples.