Structures of nucleic acids are critical to their biological activity. The secondary and tertiary structures can be adopted in response to extrinsic factors and give raise to specific functions. For RNAs structure is known to impact translation, post-translational modifications, susceptibility to degradation and binding to proteins and other ligands. Studies of the binding interaction between nucleic acids and proteins, other nucleic acids, oligonucleotides and synthetic compound may inform on structure and function as well as enable modulation of these key properties of nucleic acids, and guide discovery and development of drugs.
Microcalorimetry and biosensors are two key biophysical techniques for characterizing these binding interactions, with biosensor use expanding. Here, we give examples of how isothermal titration calorimetry (ITC) and grating coupled interferometry (GCI) can be applied in nucleic acid research, drug discovery and development.
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Structures of nucleic acids are critical to their biological activity. The secondary and tertiary structures can be adopted in response to extrinsic factors and give raise to specific functions. For RNAs structure is known to impact translation, post-translational modifications, susceptibility to degradation and binding to proteins and other ligands. Studies of the binding interaction between nucleic acids and proteins, other nucleic acids, oligonucleotides and synthetic compound may inform on structure and function as well as enable modulation of these key properties of nucleic acids, and guide discovery and development of drugs.
Microcalorimetry and biosensors are two key biophysical techniques for characterizing these binding interactions, with biosensor use expanding. Here, we give examples of how isothermal titration calorimetry (ITC) and grating coupled interferometry (GCI) can be applied in nucleic acid research, drug discovery and development.
Please download the poster to read more.
