| 00:00:00 | What is the real sensitivity of DLS for nanoparticle and protein measurements? |
| 00:01:26 | What is the real sensitivity of DLS for nanoparticle and protein measurements ? |
| 00:02:21 | Overview |
| 00:03:35 | DLS = Dynamic light scattering |
| 00:04:41 | Some Malvern references |
| 00:06:31 | Specifications – the size limits |
| 00:10:18 | Reasons for the limits |
| 00:12:14 | Prediction tools in the software |
| 00:13:05 | Minimum Concentration Calculator |
| 00:13:40 | Minimum concentration calculator example |
| 00:14:59 | Minimum concentration for lysozyme |
| 00:15:50 | Application Note: Lysozyme at 0.1 mg/mL |
| 00:18:30 | Application: Lysozyme at 0.1 mg/mL [µV] |
| 00:20:59 | Concentration and Scattering Calculator |
| 00:21:47 | Concentration and scattering example |
| 00:24:00 | Concentration and scattering, typical output |
| 00:26:27 | Which calculator is “better”? |
| 00:27:25 | Application Note: Sucrose |
| 00:28:37 | Application Note: Sucrose |
| 00:30:26 | Application Note: Cholesterol |
| 00:31:49 | Application Note: Aluminum Polyoxocations |
| 00:33:19 | Hints for low size measurements |
| 00:35:18 | Application Note: Polystyrene Latex 4µm |
| 00:36:27 | Large Particles: Backscattering is Better |
| 00:37:54 | Application Note: Polystyrene Latex 4µm |
| 00:39:10 | Hints for large size measurements |
| 00:41:28 | For performance at the limits, review tips |
| 00:42:27 | Practical Advice |
| 00:44:13 | Thank you! |
| 00:45:07 | Contact Information |
In the Zetasizer Nano software, modeling tools can predict the minimum concentration for DLS measurements. However, close to the limit of detectability, sample preparation and system set up become more important in achieving good data quality