What is the real sensitivity of DLS for nanoparticle and protein measurements?

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00:00:00 What is the real sensitivity of DLS for nanoparticle and protein measurements?
00:01:26 What is the real sensitivity of DLS for nanoparticle and protein measurements ?
00:02:21 Overview
00:03:35 DLS = Dynamic light scattering
00:04:41 Some Malvern references
00:06:31 Specifications – the size limits
00:10:18 Reasons for the limits
00:12:14 Prediction tools in the software
00:13:05 Minimum Concentration Calculator
00:13:40 Minimum concentration calculator example
00:14:59 Minimum concentration for lysozyme
00:15:50 Application Note: Lysozyme at 0.1 mg/mL
00:18:30 Application: Lysozyme at 0.1 mg/mL [µV]
00:20:59 Concentration and Scattering Calculator
00:21:47 Concentration and scattering example
00:24:00 Concentration and scattering, typical output
00:26:27 Which calculator is “better”?
00:27:25 Application Note: Sucrose
00:28:37 Application Note: Sucrose
00:30:26 Application Note: Cholesterol
00:31:49 Application Note: Aluminum Polyoxocations
00:33:19 Hints for low size measurements
00:35:18 Application Note: Polystyrene Latex 4µm
00:36:27 Large Particles: Backscattering is Better
00:37:54 Application Note: Polystyrene Latex 4µm
00:39:10 Hints for large size measurements
00:41:28 For performance at the limits, review tips
00:42:27 Practical Advice
00:44:13 Thank you!
00:45:07 Contact Information
In the Zetasizer Nano software, modeling tools can predict the minimum concentration for DLS measurements. However, close to the limit of detectability, sample preparation and system set up become more important in achieving good data quality