日期记录: July 15 2021
Viral vectors, such as Recombinant Adeno-Associated Viruses (rAAV) Adenoviruses (AV) are increasingly being used in the development of gene therapies and vaccines. The efficient development of viral vectors order to develop effective therapies and vaccines presents a number of challenges that we will address in this webinar.
Viral vectors are typically produced in engineered cell lines in large bioreactors and then isolated from the bulk harvest through cell-lysis and multiple rounds of purification for example, via density gradient centrifugation, ion-exchange chromatography, and tangential flow filtration. Multiple assays are performed during this process to determine key analytical attributes for the determination of yield, efficacy, and safety, as well as to enable process understanding and control. For AAV vectors, these parameters include capsid/particle size and count, often measured using assays such as Elisa, AUC and TEM.
This webinar discusses the use of Multi-angle Dynamic Light Scattering (MADLS) and Nanoparticle Tracking Analysis as label-free 5-minute methods to assess capsid yield and aggregation across the various viral vector processing steps. Real world examples will be elaborated on, including the use of MADLS to reduce the development time of rAAV-vectored gene therapies. Bringing effective, stable vaccines and therapeutics to market in a rapid and cost-effective manner has never been more important, and we look forward to using this time to describe how Malvern Panalytical delivers the information essential to this process in a simple and efficient manner.
Join our free MasterClass series on gene therapy and viral vectors analysis
- Webinar 1: Comprehensive viral vector characterization: The value of rapid label-free biophysical techniques More info
- Webinar 2: Quantifying viral vector quality attributes with MADLS – How to remove analytical roadblocks from my gene therapy or vaccine process development pathway
- Weinar 3: Optimising loading time and stability of Virally Vectored therapies and vaccines using SEC and DSC. More info
Interested to learn about other techniques and their applications deployed in pharmaceuticals?
These include X-ray diffraction for polymorphic phase analysis, Laser diffraction for particle size analysis and much more? Read our entire summary here