This CCM guide demonstrates how to set up the dilution factor, and how to change it post-data collection, for your NanoSight instrument.
On your NanoSight instrument, the sample must be diluted to a number concentration of between 10e8 and 10e9 particles per milliliter (depending on particle type) and not contain particles larger than 1 um diameter. Such particles will degrade image quality and may sediment in the sample chamber necessitating frequent cleaning of the optical surfaces. When viewed within NTA software for NanoSight, the optimum number of particles in the field of view is 20-60.
The choice of diluent is important and should be particle-free. This should be checked within the NanoSight instrument prior to use. Should particles be present the diluent should be changed or filtered through one or more compatible membranes, ending with a 20 nm pore size. The diluent should not adversely affect particle dispersion, conformation, or sample stability i.e. it should not induce unwanted flocculation, aggregation, or disassembly. Should the sample require dispersion before analysis, gentle agitation can be used, although the introduction of bubbles into the sample will degrade image quality (bubbles are very effective light scattering centers).
This CCM guide demonstrates how to set up the dilution factor, and how to change it post-data collection, for your NanoSight instrument.
