| 00:00:00 | Welcome! |
| 00:01:22 | Using NTA to study nanoparticle diffusion |
| 00:01:56 | NanoSight’s Technology |
| 00:02:55 | Nanoparticle Tracking Analysis |
| 00:03:26 | Principle of measurements |
| 00:03:58 | NTA instruments |
| 00:04:20 | Information |
| 00:05:20 | Light scattering vs fluorescence mode |
| 00:06:36 | Diffusion of nanomaterials in liquid media |
| 00:09:12 | Importance of diffusion in drug delivery |
| 00:11:14 | Synthesis of functionalised silica nanoparticles |
| 00:11:31 | Thiolated silica nanoparticles as a model system |
| 00:12:37 | DLS and TEM |
| 00:14:12 | Surface functionalisation |
| 00:15:40 | Sizes of nanoparticles before (1) and after PEGylation (2-4) |
| 00:16:45 | Small angle neutron scattering |
| 00:17:22 | Diffusion of functionalised silica in polymer solutions |
| 00:17:28 | Diffusion of thiolated silica in polymer solutions |
| 00:19:55 | Diffusion of PEGylated silica in polymer solutions |
| 00:21:07 | Diffusion of thiolated and PEGylated nanoparticles in PAA solutions of different pH |
| 00:23:17 | Diffusion of PEGylated nanoparticles in PAA and PEG |
| 00:24:28 | Diffusion of functionalised silica in mucin dispersions and mucus gels |
| 00:25:24 | Mucosal adhesion and penetration of nanoparticles |
| 00:25:57 | Diffusion of nanoparticles in porcine gastric mucin dispersions |
| 00:26:59 | Diffusion coefficients at different temperatures |
| 00:27:35 | Permeation of nanoparticles through freshly excised porcine gastric mucosa |
| 00:29:38 | Nanoparticles functionalised with different alkyne terminated poly(2-alkyl-2-oxazolines) |
| 00:30:11 | Characterisation |
| 00:30:48 | Diffusion in mucus studied using NTA |
| 00:32:02 | Ex vivo mucosa penetration |
| 00:33:04 | Ex vivo mucosal penetration |
| 00:33:37 | Effect of Particle Surface Chemistry on Mucosal Penetration |
| 00:34:26 | Conclusions |
| 00:35:31 | Acknowledgements |
| 00:37:32 | Thank you for your attentionAny questions? |
| 00:43:27 | Contact Information and Thank You |
• Nanoparticle Tracking Analysis (NTA) was used to study the diffusion of silica nanoparticles functionalized with different polymers in aqueous solutions of water-soluble polymers and in dispersions of porcine gastric mucin
• It was found that the diffusivity of nanoparticles is affected by their dimensions, medium viscosity, and specific interactions between nanoparticles and macromolecules in solution. Strong attractive interactions such as hydrogen bonding were found to hamper diffusion.
• This study demonstrated that decoration of nanoparticle surfaces with poly(2-ethyl-2-oxazoline) or poly(ethylene glycol) enhances their ability to travel through mucus. The diffusion results generated by NTA were in good agreement with the data on the permeation of these nanoparticles through ex vivo porcine stomach mucus.
• It was found that the diffusivity of nanoparticles is affected by their dimensions, medium viscosity, and specific interactions between nanoparticles and macromolecules in solution. Strong attractive interactions such as hydrogen bonding were found to hamper diffusion.
• This study demonstrated that decoration of nanoparticle surfaces with poly(2-ethyl-2-oxazoline) or poly(ethylene glycol) enhances their ability to travel through mucus. The diffusion results generated by NTA were in good agreement with the data on the permeation of these nanoparticles through ex vivo porcine stomach mucus.